Heme Oxygenase 1(HO-1) Recombinant Rabbit Monoclonal Antibody [SP08-07] (ET1604-45)

CITATION

APPLICATIONS

  • WB
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Heme Oxygenase 1(HO-1) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-45, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

PHD1/prolyl hydroxylase Recombinant Rabbit Monoclonal Antibody [SP00-48] (ET1604-5)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of PHD1/prolyl hydroxylase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1604-5, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: PC-12 cell lysate
Lane 2: NIH/3T3 cell lysate

PMS2 Recombinant Rabbit Monoclonal Antibody [SY08-09] (ET1605-1)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
Western blot analysis of PMS2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: MCF-7 cell lysate
Lane 2: Hela cell lysate

VEGF Receptor 1 Recombinant Rabbit Monoclonal Antibody [SY09-09] (ET1605-11)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of VEGF Receptor 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: human lung tissue lysate
Lane 2: mouse lung tissue lysate

p53 Recombinant Rabbit Monoclonal Antibody [SY010-6] (ET1605-16)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
Western blot analysis of p53 on 293 cells lysates using anti-p53 antibody at 1/1,000 dilution.

Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody [SY88-19] (ET1605-17)

APPLICATIONS

  • WB
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of Cytokeratin 5 on A431 cell lysates using anti-Cytokeratin 5 at 1/500 dilution.

TACC3 Recombinant Rabbit Monoclonal Antibody [SY10-06] (ET1605-18)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of TACC3 on different lysates using anti-TACC3 antibody at 1/1,000 dilution.
Positive control:   
Lane 1: HT29                
Lane 2: Hela

PDGF Receptor beta Recombinant Rabbit Monoclonal Antibody [SY10-08] (ET1605-20)

APPLICATIONS

  • WB
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of PDGF Receptor beta on NIH/3T3 cell lysates using anti-PDGF Receptor beta antibody at 1/1,000 dilution.

ALDH1A1 Recombinant Rabbit Monoclonal Antibody [SY11-02] (ET1605-24)

APPLICATIONS

  • WB
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of ALDH1A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: mouse lung tissue lysate
Lane 2: human liver tissue lysate
Lane 3: human kidney tissue lysate

FUBP1 Recombinant Rabbit Monoclonal Antibody [SY11-04] (ET1605-26)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of FUBP1 on different lysates using anti-FUBP1 antibody at 1/1,000 dilution.
Positive control:   
Lane 1: Hela                 
Lane 2: Raji

SHIP Recombinant Rabbit Monoclonal Antibody [SY11-08] (ET1605-30)

APPLICATIONS

  • WB
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
Western blot analysis of SHIP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: HL-60 cell lysate
Lane 2: Daudi cell lysate

CD20 Recombinant Rabbit Monoclonal Antibody [SY12-01] (ET1605-33)

APPLICATIONS

  • WB
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MONKEY
Western blot analysis of CD20 on Raji cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

HDAC1 Recombinant Rabbit Monoclonal Antibody [SY12-04] (ET1605-35)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of HDAC1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-35, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 2: MCF-7 cell lysate
Lane 2: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 2: K562 cell lysate

PCNA Recombinant Rabbit Monoclonal Antibody [SY12-07] (ET1605-38)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of PCNA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: 293 cell lysate
Lane 3: A431 cell lysate

CDX2 Recombinant Rabbit Monoclonal Antibody [SY09-02] (ET1605-4)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • RAT
  • RABBIT
Western blot analysis of CDX2 on AGS cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

YY1 Recombinant Rabbit Monoclonal Antibody [SY29-01] (ET1605-40)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of YY1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 3: HL-60 cell lysate
Lane 4: Daudi cell lysate
Lane 5: THP-1 cell lysate

V5 tag Recombinant Rabbit Monoclonal Antibody [SY30-01] (ET1605-41)

APPLICATIONS

  • WB

REACTIVITY

Western blot analysis of V5 tag on V5-tagged recombinant protein lysates using anti-V5 tag antibody at 1/1,000 dilution.

STAT3 Recombinant Rabbit Monoclonal Antibody [SY34-01] (ET1605-45)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of STAT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: MCF-7 cell lysate
Lane 2: SiHa cell lysate
Lane 3: A549 cell lysate

STAT6 Recombinant Rabbit Monoclonal Antibody [SY13-09] (ET1605-49)

APPLICATIONS

  • WB
  • ICC
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of STAT6 on different lysates using anti-STAT6 antibody at 1/1,000 dilution.
  Positive control: 
  Lane 1: NIH/3T3 
  Lane 2: Hela

Phospho-Smad5 (S463/465) Recombinant Rabbit Monoclonal Antibody [SY09-03] (ET1605-5)

APPLICATIONS

  • WB
  • ICC
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Phospho-Smad5(S463/465) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-5, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: mouse brain tissue lysate
Lane 2: rat brain tissue lysate
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