Tenascin C Recombinant Rabbit Monoclonal Antibody [SU36-01] (ET1608-50)

APPLICATIONS

  • WB
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Tenascin C antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-50, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Phospho-RSK1 p90 (T359+S363) Recombinant Rabbit Monoclonal Antibody [SU03-65] (ET1608-53)

APPLICATIONS

  • WB
  • ICC
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Phospho-RSK1 p90(T359+S363) on different lysates using anti-Phospho-RSK1 p90(T359+S363) antibody at 1/1,000 dilution.
Positive control: 
  Lane 1: A431 
  Lane 2: 293 
  Lane 3: MCF-7

ASK1 Recombinant Rabbit Monoclonal Antibody [SU36-06] (ET1608-54)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of ASK1 on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-54, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

PARP Recombinant Rabbit Monoclonal Antibody [SU03-68] (ET1608-56)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of PARP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-56, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: A549 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Hela cell lysate

Lactate Dehydrogenase Recombinant Rabbit Monoclonal Antibody [SU39-06] (ET1608-57)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
  • ZEBRAFISH
Western blot analysis of Lactate Dehydrogenase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: A549 cell lysate
Lane 3: MCF-7 cell lysate

Phospho-PAK1(S144)+PAK2(S141)+PAK3(S139) Recombinant Rabbit Monoclonal Antibody [SU03-70] (ET1608-58)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Phospho-PAK1(S144)+PAK2(S141)+PAK3(S139) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-58, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 2: SH-SY5Y cell lysate

Calreticulin Recombinant Rabbit Monoclonal Antibody [SU37-03] (ET1608-60)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Calreticulin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: SH-SY5Y cell lysate
Lane 2: HL-60 cell lysate

p27 KIP 1 Recombinant Rabbit Monoclonal Antibody [SU37-04] (ET1608-61)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • RAT
ICC staining of p27 KIP 1 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-61, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

p16INK4A Recombinant Rabbit Monoclonal Antibody [SU0702] (ET1608-62)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
Western blot analysis of p16INK4A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-62, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: 293 cell lysate
Lane 2: SiHa cell lysate

active+pro Caspase-3 Recombinant Rabbit Monoclonal Antibody [SU38-04] (ET1608-64)

APPLICATIONS

  • WB
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
Western blot analysis of active+pro Caspase-3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-64, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Jurkat cell lysate
Lane 2: NIH/3T3 cell lysate
Lane 3: human lung tissue lysate

Phospho-eIF4E (S209) Recombinant Rabbit Monoclonal Antibody [SU0396] (ET1608-66)

APPLICATIONS

  • WB
  • ICC
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Phospho-eIF4E (S209) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-66, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: mouse spleen tissue lysate
Lane 2: rat spleen tissue lysate

Myosin light chain kinase Recombinant Rabbit Monoclonal Antibody [SU40-06] (ET1608-68)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Myosin light chain kinase on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-68, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Caspase-1 Recombinant Rabbit Monoclonal Antibody [SU40-07] (ET1608-69)

CITATION

APPLICATIONS

  • WB
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Caspase-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-69, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: THP-1 cell lysate
Lane 2: Jurkat cell lysate

PI 3 Kinase p85 alpha Recombinant Rabbit Monoclonal Antibody [SU04-07] (ET1608-70)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of PI 3 Kinase p85 alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-70, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: MCF-7 cell lysate
Lane 2: Raji cell lysate

Acetyl-Histone H3 (K56) Recombinant Rabbit Monoclonal Antibody [SU30-10] (ET1608-9)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Histone H3(acetyl K56) on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-9, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

MCSF Recombinant Rabbit Monoclonal Antibody [SU0413] (ET1609-1)

APPLICATIONS

  • WB
  • IHC-P
  • ICC
  • IP

REACTIVITY

  • HUMAN
Western blot analysis of MCSF on mouse colon tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

YB1 Recombinant Rabbit Monoclonal Antibody [ST0432] (ET1609-10)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of YB1 on different lysates using anti-YB1 antibody at 1/1,000 dilution.
Positive control:   
Lane 1: Hela             
Lane 2: Jurkat

SOX18 Recombinant Rabbit Monoclonal Antibody [ST43-02] (ET1609-11)

APPLICATIONS

  • WB
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of SOX18 on K562 cells lysates using anti-SOX18 antibody at 1/1,000 dilution.

Phospho-p53 (T55) Recombinant Rabbit Monoclonal Antibody [ST0439] (ET1609-13)

APPLICATIONS

  • WB

REACTIVITY

  • HUMAN
Western blot analysis of Phospho-p53 (T55) on human skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Phospho-p53 (S376) Recombinant Rabbit Monoclonal Antibody [ST0440] (ET1609-14)

CITATION

APPLICATIONS

  • WB

REACTIVITY

  • HUMAN
Western blot analysis of Phospho-p53(S376) on human skin lysates using anti-Phospho-p53(S376) antibody at 1/1,000 dilution.
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