STAT6 Recombinant Rabbit Monoclonal Antibody [SY02-72] (ET1607-61)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of STAT6 on different lysates using anti-STAT6 antibody at 1/1,000 dilution.
Positive control:   
Lane 1: Hela                 
Lane 2: Raji

MKLP1 Recombinant Rabbit Monoclonal Antibody [SY02-74] (ET1607-63)

APPLICATIONS

  • WB
  • ICC
  • IF

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of MKLP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: 293 cell lysate
Lane 2: A549 cell lysate

HAPLN1 Recombinant Rabbit Monoclonal Antibody [SY02-17] (ET1607-7)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
  • ZEBRAFISH
Western blot analysis of HAPLN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-7, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: BT-20 cell lysate
Lane 2: HUVEC cell lysate
Lane 3: SW480 cell lysate

GSK3 beta Recombinant Rabbit Monoclonal Antibody [SY28-03] (ET1607-71)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of GSK3 beta on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-71, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: 293 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: PC-12 cell lysate

Phospho-Akt1 (Ser473) Recombinant Rabbit Monoclonal Antibody [SY28-05] (ET1607-73)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of Phospho-Akt1(Ser473) on PC-3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-73, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

PI 3 Kinase Class 3 Recombinant Rabbit Monoclonal Antibody [SY0286] (ET1607-74)

APPLICATIONS

  • WB
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of PI 3 Kinase Class 3 on C2C12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

E-Cadherin Recombinant Rabbit Monoclonal Antibody [SY0287] (ET1607-75)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
Western blot analysis of E-Cadherin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-75, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: A431 cell lysate
Lane 2: MCF-7 cell lysate

HDAC2 Recombinant Rabbit Monoclonal Antibody [SY29-02] (ET1607-78)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of HDAC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-78, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: 293T cell lysate
Lane 2: K562 cell lysate

Plectin Recombinant Rabbit Monoclonal Antibody [SY29-04] (ET1607-80)

APPLICATIONS

  • WB
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Plectin antibody. Counter stained with hematoxylin.

Rb Recombinant Rabbit Monoclonal Antibody [SY63-03] (ET1607-9)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of Rb on mouse lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-9, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Cleaved PARP Recombinant Rabbit Monoclonal Antibody [SU0314] (ET1608-10)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IP
  • FC

REACTIVITY

  • HUMAN
Western blot analysis of Cleaved PARP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-10, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Jurkat cell lysate
Lane 2: A549 cell lysate

Phospho-HSF1 (S326) Recombinant Rabbit Monoclonal Antibody [SU31-03] (ET1608-11)

APPLICATIONS

  • WB
  • ICC
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
Western blot analysis of Phospho-HSF1(S326) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: BT-20 cell lysate
Lane 3: MCF-7 cell lysate

Bim Recombinant Rabbit Monoclonal Antibody [SU0318] (ET1608-14)

APPLICATIONS

  • WB
  • ICC
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
Western blot analysis of Bim on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-14, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Raji cell lysate
Lane 2: Jurkat cell lysate
Lane 3: A431 cell lysate

PKC alpha Recombinant Rabbit Monoclonal Antibody [SU31-08] (ET1608-15)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of PKC alpha on different lysates using anti-PKC alpha antibody at 1/1,000 dilution.
Positive control: 
  Lane 1: K562 
  Lane 2: 293

Caldesmon Recombinant Rabbit Monoclonal Antibody [SU03-19] (ET1608-16)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Caldesmon on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-16, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

LGR5/GPR49 Recombinant Rabbit Monoclonal Antibody [SU32-04] (ET1608-18)

APPLICATIONS

  • WB
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of LGR5/GPR49 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: mouse spinal cord tissue lysate
Lane 2: human skeletal muscle tissue lysate

ELK1 Recombinant Rabbit Monoclonal Antibody [SU32-05] (ET1608-19)

APPLICATIONS

  • WB
  • ICC
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of ELK1 on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-19, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

ABCG1 Recombinant Rabbit Monoclonal Antibody [SU03-26] (ET1608-20)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
  • RAT
Western blot analysis of ABCG1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: THP-1 cell lysate
Lane 2: MCF-7 cell lysate

Bak Recombinant Rabbit Monoclonal Antibody [SU32-07] (ET1608-21)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
Western blot analysis of Bak on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: human skeletal muscle tissue lysate
Lane 3: AGS cell lysate

Phospho-IRF3 (S386) Recombinant Rabbit Monoclonal Antibody [SU03-28] (ET1608-22)

APPLICATIONS

  • WB
  • ICC
  • IF

REACTIVITY

  • HUMAN
ICC staining of Phospho-IRF3(S386) in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-22, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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