Phospho-Rb (S807) Recombinant Rabbit Monoclonal Antibody [SR3-82] (ET1602-36)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Phospho-Rb(S807) on A549 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-36, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

IFNAR1 Recombinant Rabbit Monoclonal Antibody [SR45-08] (ET1602-37)

APPLICATIONS

  • WB
  • IP
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of IFNAR1 on SiHa cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Acetyl-p53 (K370) Recombinant Rabbit Monoclonal Antibody [SR40-09] (ET1602-38)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of p53(acetyl K370) on HepG2 cell lysates using anti-p53(acetyl K370) antibody at 1/1,000 dilution.
Positive control: 
Lane 1: HepG2 treated with Etoposide 20uM and Trichostatin A 500 nM for 6 hours whole cell lysates 
Lane 2: HepG2 untreated whole cell lysates

Caspase-3 Recombinant Rabbit Monoclonal Antibody [SR03-01] (ET1602-39)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • RAT
Western blot analysis of Caspase-3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-39, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 2: 293 cell lysate

beta Tubulin Recombinant Rabbit Monoclonal Antibody [SR25-04] (ET1602-4)

APPLICATIONS

  • WB
  • ICC
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of beta Tubulin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: A431 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: mouse brain tissue lysate
Lane 5: Hela cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: PC-12 cell lysate

Acetyl-Histone H4 (K5) Recombinant Rabbit Monoclonal Antibody [SR31-07] (ET1602-40)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Histone H4(acetyl K5) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: CRC cell lysate
Lane 2: Hela cell lysate
Lane 3: SH-SY5Y cell lysate

Mono+Di-Methyl-Histone H3 (K79) Recombinant Rabbit Monoclonal Antibody [SR42-06] (ET1602-41)

APPLICATIONS

  • WB
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Histone H3(mono+di+methyl K79) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: mouse testis tissue lysate
Lane 2: CRC cell lysate"

MMP12 Recombinant Rabbit Monoclonal Antibody [SR03-23] (ET1602-42)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of MMP12 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-42, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: A549 cell lysate
Lane 2: Hela cell lysate
Lane 3: MCF-7  cell lysate
Lane 4: THP-1 cell lysate

Survivin Recombinant Rabbit Monoclonal Antibody [SR44-02] (ET1602-43)

APPLICATIONS

  • WB
  • IHC-P
  • IP

REACTIVITY

  • MOUSE
  • RAT
Western blot analysis of Survivin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-43, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: L929 cell lysate
Lane 2: F9 cell lysate

Firefly Luciferase Recombinant Rabbit Monoclonal Antibody [SR03-86] (ET1602-44)

APPLICATIONS

  • WB
  • ICC
  • IF

REACTIVITY

  • FIREFLY
Western blot analysis of Firefly Luciferase   on luciferase recombinant protein lysate using anti-Firefly Luciferase antibody at 1/1,000 dilution.

Renilla Luciferase Recombinant Rabbit Monoclonal Antibody [SR07-830] (ET1602-45)

APPLICATIONS

  • WB
  • ICC
  • IF

REACTIVITY

  • SEA PANSY
Western blot analysis of Renilla Luciferase on luciferase recombinant protein lysate using anti-Renilla Luciferase antibody at 1/1,000 dilution.

Maltose Binding Protein Recombinant Rabbit Monoclonal Antibody [SR41-04] (ET1602-46)

APPLICATIONS

  • WB

REACTIVITY

  • E.COLI
Western blot analysis of Maltose Binding Protein on recombinant MBP-tag protein lysates using anti-Maltose Binding Protein antibody at 1/1,000 dilution.

Active Caspase-3 Recombinant Rabbit Monoclonal Antibody [SR01-02] (ET1602-47)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
  • PIG
Western blot analysis of Active Caspase-3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-47, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Camptothecin (2 μM) treated Jurkat cell lysate
Lane 2: Untreated Jurkat cell lysate

Mono-Methyl-Histone H3 (K36) Recombinant Rabbit Monoclonal Antibody [SR04-20] (ET1602-48)

APPLICATIONS

  • WB
  • ICC
  • IF

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of Histone H3(mono methyl K36) on different lysates using anti-Histone H3(mono methyl K36) antibody at 1/1,000 dilution.
Positive control:   
Lane 1: Hela           
Lane 2: NIH/3T3

M6PR Recombinant Rabbit Monoclonal Antibody [SR45-09] (ET1602-5)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of M6PR on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-5, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: SiHa cell lysate
Lane 2: human skin tissue lysate

Cdk2 Recombinant Rabbit Monoclonal Antibody [SR01-01] (ET1602-6)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Cdk2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: A549 cell lysate
Lane 2: F9 cell lysate

GFP Recombinant Rabbit Monoclonal Antibody [SR48-02] (ET1602-7)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • AEQUOREA VICTORIA
Western blot analysis of GFP on recombinant GFP protein using anti-GFP  antibody at 1/5,000 dilution.

HP1 alpha Recombinant Rabbit Monoclonal Antibody [SR44-03] (ET1602-8)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of HP1 alpha on different cell lysates using anti-HP1 alpha antibody at 1/1,000 dilution.
Positive control: 
  Lane 1: MCF-7 
  Lane 2: Hela

p16 ARC Recombinant Rabbit Monoclonal Antibody [SR34-02] (ET1602-9)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of p16 ARC on different cell lysates using anti-p16 ARC antibody at 1/1,000 dilution.
Positive control: 
  Lane 1: MCF-7 
  Lane 2: SK-BR-3

Caveolin-1 Recombinant Rabbit Monoclonal Antibody [SZ02-01] (ET1603-1)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of Caveolin-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: A549 cell lysate
Lane 2: A431 cell lysate
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