CD3 epsilon Recombinant Rabbit Monoclonal Antibody [SY0239] (ET1607-29)

APPLICATIONS

  • WB
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
Western blot analysis of CD3 epsilon on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-29, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: human thymus tissue lysate
Lane 2: Jurkat cell lysate

GFP Recombinant Rabbit Monoclonal Antibody [SY0243] (ET1607-31)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IP

REACTIVITY

Western blot analysis of GFP on K562 cell lysates
 transfected with GFP. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-31, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Phospho-JAK2 (Y1007+Y1008) Recombinant Rabbit Monoclonal Antibody [SY24-03] (ET1607-34)

APPLICATIONS

  • WB
  • ICC
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
ICC staining of Phospho-JAK2(Y1007+Y1008) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

JAK2 Recombinant Rabbit Monoclonal Antibody [SY0245] (ET1607-35)

APPLICATIONS

  • WB
  • ICC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of JAK2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-35, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: A549 cell lysate
Lane 2: MCF-7 cell lysate

N Cadherin Recombinant Rabbit Monoclonal Antibody [SY02-46] (ET1607-37)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of N Cadherin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: SH-SY5Y cell lysate
Lane 2: Hela cell lysate
Lane 3: NIH/3T3 cell lysate

Stat3 Recombinant Rabbit Monoclonal Antibody [SY34-01] (ET1607-38)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
  • ZEBRAFISH
Western blot analysis of Stat3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: SiHa cell lysate
Lane 2: SW480 cell lysate
Lane 1: PC-12 cell lysate
Lane 2: SHG-44 cell lysate

NADPH oxidase 4/NOX4 Recombinant Rabbit Monoclonal Antibody [SY0214] (ET1607-4)

APPLICATIONS

  • WB
  • ICC
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of NADPH oxidase 4/NOX4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: JAR cell lysate
Lane 2: SH-SY5Y cell lysate

Phospho-STAT3 (S727) Recombinant Rabbit Monoclonal Antibody [SY24-09] (ET1607-39)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • RAT
  • MOUSE
Western blot analysis of Phospho-STAT3(S727) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-39, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: A431 cell lysate
Lane 1: NIH/3T3 cell lysate
Lane 2: Jurkat cell lysate

Smad3 Recombinant Rabbit Monoclonal Antibody [SY25-01] (ET1607-41)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Smad3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: A549 cell lysate
Lane 2: mouse spleen tissue lysate

Smad1 Recombinant Rabbit Monoclonal Antibody [SY0254] (ET1607-42)

APPLICATIONS

  • WB
  • ICC
  • IF

REACTIVITY

  • HUMAN
  • MOUSE
ICC staining of Smad1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-42, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody [SY25-03] (ET1607-43)

CITATION

APPLICATIONS

  • WB
  • IHC-P
  • FC
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
  • ZEBRAFISH
Western blot analysis of alpha smooth muscle Actin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-43, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: Hela cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: mouse heart tissue lysate

Phospho-p95/NBS1 (S343) Recombinant Rabbit Monoclonal Antibody [SY0215] (ET1607-5)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
ICC staining of Phospho-p95/NBS1 (S343) in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-5, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

TCF3 Recombinant Rabbit Monoclonal Antibody [SY0258] (ET1607-47)

APPLICATIONS

  • WB
  • FC

REACTIVITY

  • HUMAN
Flow cytometric analysis of 293 cells with TCF3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

CDK1 Recombinant Rabbit Monoclonal Antibody [SM01-44] (ET1607-51)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of CDK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Jurkat cell lysate
Lane 2: U937 cell lysate

alpha smooth muscle Actin Recombinant Rabbit Monoclonal Antibody [SY02-64] (ET1607-53)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of alpha smooth muscle Actin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: A431 cell lysate
Lane 2: Hela cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: mouse heart tissue lysate
Lane 5: mouse smooth muscle tissue lysate

Phospho-GSK3 (SK3 alpha(Y216)+SK3 beta(Y279)) Recombinant Rabbit Monoclonal Antibody [SY26-05] (ET1607-54)

APPLICATIONS

  • WB
  • ICC
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
ICC staining of Phospho-GSK3(alpha+beta)(Y216+Y279) in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-54, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

DNAJC15 Recombinant Rabbit Monoclonal Antibody [SY26-04] (ET1607-52)

APPLICATIONS

  • WB
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of DNAJC15 on human liver tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-52, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Topoisomerase Ⅱ alpha Recombinant Rabbit Monoclonal Antibody [SY27-00] (ET1607-59)

APPLICATIONS

  • WB
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Topoisomerase Ⅱ alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-59, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: mouse testis tissue lysate
Lane 2: MCF-7 cell lysate

VASP Recombinant Rabbit Monoclonal Antibody [SY02-16] (ET1607-6)

APPLICATIONS

  • WB
  • IP

REACTIVITY

  • HUMAN
Western blot analysis of VASP on different lysates using anti-VASP antibody at 1/1,000 dilution.
Positive control:   
Lane 1: Hela                 
Lane 2: MCF-7 
Lane 3: HT29

Phospho-GSK3 beta (Ser 9) Recombinant Rabbit Monoclonal Antibody [SY02-71] (ET1607-60)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
Western blot analysis of Phospho-GSK3 beta(Ser 9) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: A549 cell lysate
Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17