IKB alpha Recombinant Rabbit Monoclonal Antibody [SZ00-07] (ET1603-6)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of IKB alpha on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: NIH/3T3 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Hela cell lysate

Cytokeratin 18 Recombinant Rabbit Monoclonal Antibody [SZ80-07] (ET1603-8)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Cytokeratin 18 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-8, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: A431 cell lysate
Lane 2: human lung tissue lysate

SATB1 Recombinant Rabbit Monoclonal Antibody [SP05-03] (ET1604-10)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of SATB1 on human thymus lysates using anti-SATB1 antibody at 1/1,000 dilution.

FOXO3A Recombinant Rabbit Monoclonal Antibody [SP0054] (ET1604-11)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF

REACTIVITY

  • HUMAN
Western blot analysis of FOXO3A on different lysates using anti- FOXO3A antibody at 1/1,000 dilution.
Positive control:   
Lane 1: NIH/3T3          
Lane 2: PC-12 
Lane 3: MCF-7

Smad4 Recombinant Rabbit Monoclonal Antibody [SP05-05] (ET1604-12)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Smad4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1604-12, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: PC-12 cell lysate
Lane 2: SH-SY5Y cell lysate

ERK1 Recombinant Rabbit Monoclonal Antibody [SP05-09] (ET1604-16)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution.
Positive control:   
Lane 1: Hela               
Lane 2: Jurkat 
Lane 3: K562

Tubulin beta-III Recombinant Rabbit Monoclonal Antibody [SP06-00] (ET1604-17)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Tubulin beta-III on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1604-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: PC-12 cell lysate
Lane 2: Hela cell lysate
Lane 3: SH-SY5Y cell lysate

GFP Recombinant Rabbit Monoclonal Antibody [SP00-68] (ET1604-25)

APPLICATIONS

  • WB
  • IHC-P
  • ICC
  • IF

REACTIVITY

Western blot analysis of GFP on K562 cell lysates
 transfected with GFP. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1604-25, 1/50,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

ERG Recombinant Rabbit Monoclonal Antibody [SP06-04] (ET1604-21)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • CHIP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of ERG on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1604-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Smad2 Recombinant Rabbit Monoclonal Antibody [SP06-05] (ET1604-22)

APPLICATIONS

  • WB
  • ICC
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Smad2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1604-22, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: A431 cell lysate
Lane 3: PC-12 cell lysate

GFP Recombinant Rabbit Monoclonal Antibody [SP069-0] (ET1604-26)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

Western blot analysis of GFP on K562 cell lysates
 transfected with GFP. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1604-26, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Phospho-NF-kB p65 (S529) Recombinant Rabbit Monoclonal Antibody [SP07-00] (ET1604-27)

APPLICATIONS

  • WB
  • IP
  • FC

REACTIVITY

  • HUMAN
Flow cytometric analysis of Phospho-NF-kB p65(S529) was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1604-27, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

VEGF Recombinant Rabbit Monoclonal Antibody [SP07-01] (ET1604-28)

CITATION

APPLICATIONS

  • ICC
  • IF
  • IHC-P
  • IP
  • FC
  • WB

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of VEGF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1604-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: A431 cell lysate

Olig2 Recombinant Rabbit Monoclonal Antibody [SP07-02] (ET1604-29)

APPLICATIONS

  • WB
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Olig2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1604-29, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: HL-60 cell lysate
Lane 2: THP-1 cell lysate

ERK1 Recombinant Rabbit Monoclonal Antibody [SP05-09] (ET1604-32)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of ERK1 on different lysates using anti-ERK1 antibody at 1/1,000 dilution.
Positive control:   
Lane 1: Hela               
Lane 2: Jurkat 
Lane 3: K562

MSH6 Recombinant Rabbit Monoclonal Antibody [SP08-02] (ET1604-39)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of MSH6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1604-39, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: A549 cell lysate
Lane 2: HepG2 cell lysate

Survivin Recombinant Rabbit Monoclonal Antibody [SP07-06] (ET1604-34)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
ICC staining of Survivin in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1604-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

RNF20 Recombinant Rabbit Monoclonal Antibody [SP00-47] (ET1604-4)

APPLICATIONS

  • WB
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of RNF20 on different lysates using anti-RNF20 antibody at 1/1,000 dilution.
Positive control:   
Lane 1: Hela            
Lane 2: MCF-7 
Lane 3: Jurkat

MLH1 Recombinant Rabbit Monoclonal Antibody [SP08-04] (ET1604-41)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of MLH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1604-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Daudi cell lysate
Lane 2: K562 cell lysate
Lane 1: A431 cell lysate
Lane 2: HL-60 cell lysate

EGFR Recombinant Rabbit Monoclonal Antibody [SP00-86] (ET1604-44)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of EGFR on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1604-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: human skin tissue lysate
Lane 2: 293T cell lysate
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