DARPP32 Recombinant Rabbit Monoclonal Antibody [SU0329] (ET1608-23)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of DARPP32 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-23, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate

FOXO1A Recombinant Rabbit Monoclonal Antibody [SU33-01] (ET1608-25)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
  • ZEBRAFISH
Western blot analysis of FOXO1A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-25, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: NIH/3T3 cell lysate

EpCAM Recombinant Rabbit Monoclonal Antibody [SU03-32] (ET1608-26)

APPLICATIONS

  • WB
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of EpCAM on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: HCT116 cell lysate
Lane 2: SW480 cell lysate

Cyclin B1 Recombinant Rabbit Monoclonal Antibody [SU33-03] (ET1608-27)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
Western blot analysis of Cyclin B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: Daudi cell lysate
Lane 3: K562 cell lysate

Phospho-Nrf2 (S40) Recombinant Rabbit Monoclonal Antibody [SU0334] (ET1608-28)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
Western blot analysis of Phospho-Nrf2(S40) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: HepG2 cell lysate
Lane 2: Raji cell lysate

c-Jun Recombinant Rabbit Monoclonal Antibody [SY0298] (ET1608-3)

APPLICATIONS

  • WB
  • ICC
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of c-Jun on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-3, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: Human skin tissue lysate

YAP1 Recombinant Rabbit Monoclonal Antibody [SU33-06] (ET1608-30)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
Western blot analysis of YAP1 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

VEGF Receptor 2 Recombinant Rabbit Monoclonal Antibody [SU03-42] (ET1608-33)

APPLICATIONS

  • WB
  • ICC
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
ICC staining VEGF Receptor 2 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Cytokeratin 8 Recombinant Rabbit Monoclonal Antibody [SU0338] (ET1608-32)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of Cytokeratin 8 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-32, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: A431 cell lysate

B Raf Recombinant Rabbit Monoclonal Antibody [SU34-04] (ET1608-36)

APPLICATIONS

  • WB
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-B Raf antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Phospho-c-Jun (S63) Recombinant Rabbit Monoclonal Antibody [SY0297] (ET1608-4)

APPLICATIONS

  • WB
  • ICC
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Phospho-c-Jun(S63) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: 293T cell lysate
Lane 2: NIH/3T3 cell lysate

5T4 Recombinant Rabbit Monoclonal Antibody [SU34-05] (ET1608-37)

APPLICATIONS

  • WB
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of 5T4 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-37, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

TrkA Recombinant Rabbit Monoclonal Antibody [JJ084-04] (ET1608-44)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of TrkA on different cell lysates using anti-TrkA antibody at 1/1,000 dilution.
Positive control:   
Lane 1: Mouse brain     
Lane 2: SH-SY-5Y

AMPK alpha 1 Recombinant Rabbit Monoclonal Antibody [SU03-48] (ET1608-40)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of AMPK alpha 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: MCF-7 cell lysate
Lane 2: Hela cell lysate

CD90 Recombinant Rabbit Monoclonal Antibody [SU35-07] (ET1608-46)

APPLICATIONS

  • WB
  • IHC-P
  • ICC

REACTIVITY

  • HUMAN
  • RAT
Western blot analysis of CD90 on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

beta 2 Microglobulin Recombinant Rabbit Monoclonal Antibody [SU35-05] (ET1608-45)

APPLICATIONS

  • WB
  • ICC
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of beta 2 Microglobulin on different lysates using anti-beta 2 Microglobulin antibody at 1/1,000 dilution.
Positive control:   
Lane 1: Raji                
Lane 2: U937

CD31 Recombinant Rabbit Monoclonal Antibody [SU03-59] (ET1608-48)

CITATION

APPLICATIONS

  • WB
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
Western blot analysis of CD31 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: THP-1 cell lysate
Lane 2: human placenta tissue lysate

CaMKⅡ Recombinant Rabbit Monoclonal Antibody [SU03-57] (ET1608-47)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of CaMKⅡ on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-47, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: SH-SY-5Y cell lysate
Lane 2: PC-12 cell lysate
Lane 3: SHG-44 cell lysate

Cathepsin D Recombinant Rabbit Monoclonal Antibody [SU0360] (ET1608-49)

APPLICATIONS

  • WB
  • ICC
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of Cathepsin D on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: A549 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: SK-Br-3 cell lysate

mTOR Recombinant Rabbit Monoclonal Antibody [SU30-00] (ET1608-5)

CITATION

APPLICATIONS

  • WB
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of mTOR on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-5, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: mouse testis tissue lysate
Lane 2: SiHa cell lysate
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