cl-2 Recombinant Rabbit Monoclonal Antibody [SZ10-03] (ET1603-11)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of Bcl-2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Jurkat cell lysate
Lane 2: U937 cell lysate
Lane 3: THP-1 cell lysate

NF-kB p65 Recombinant Rabbit Monoclonal Antibody [SZ10-04] (ET1603-12)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • ZEBRAFISH
Western blot analysis of NF-κB p65 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-12, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Jurkat cell lysate
Lane 2: K562 cell lysate
Lane 1: THP-1 cell lysate
Lane 1: MCF-7 cell lysate
Lane 2: Hela cell lysate

ATF2 Recombinant Rabbit Monoclonal Antibody [SZ17-01] (ET1603-15)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
ICC staining ATF2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Phospho-eIF-2a (S51) Recombinant Rabbit Monoclonal Antibody [SZ01-06] (ET1603-14)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Phospho-eIF-2a(S51) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-14, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: HUVEC cell lysate
Lane 2: Hela cell lysate
Lane 2: PC-3M cell lysate

Caspase-8 Recombinant Rabbit Monoclonal Antibody [SZ01-08] (ET1603-16)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
Western blot analysis of Caspase-8 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-16, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Jurkat cell lysate
Lane 2: K562 cell lysate

NFkB p105/p50 Recombinant Rabbit Monoclonal Antibody [SZ20-01] (ET1603-18)

APPLICATIONS

  • WB
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of NFkB p105/p50 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: PC-12 cell lysate

Tau Recombinant Rabbit Monoclonal Antibody [SZ03-03] (ET1603-2)

APPLICATIONS

  • WB
  • IHC-P
  • IP

REACTIVITY

  • MOUSE
  • RAT
  • HUMAN
Western blot analysis of Tau on mouse brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

MEK1 Recombinant Rabbit Monoclonal Antibody [SZ22-01] (ET1603-20)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
  • COW
  • DOG
Western blot analysis of MEK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: A431 cell lysate
Lane 2: HepG2 cell lysate 
Lane 3: Hela cell lysate

Phospho-Erk1(T202)+Erk2(T185) Recombinant Rabbit Monoclonal Antibody [SZ2-4] (ET1603-22)

APPLICATIONS

  • WB
  • ICC
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
ICC staining of Phospho-Erk1(T202)+Erk2(T185) in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-22, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ERK2 Recombinant Rabbit Monoclonal Antibody [SZ25-01] (ET1603-23)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of ERK2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-23, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: PC-12 cell lysate
Lane 2: Hela cell lysate

Phospho-c-Myc (T58+S62) Recombinant Rabbit Monoclonal Antibody [SZ02-06] (ET1603-24)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IP

REACTIVITY

  • HUMAN
  • RAT
Western blot analysis of Phospho-c-Myc (T58+S62) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: K562 cell lysate
Lane 2: SH-SY5Y cell lysate

pro Caspase-3 Recombinant Rabbit Monoclonal Antibody [SZ02-08] (ET1603-26)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of pro Caspase-3 on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Caspase-9 Recombinant Rabbit Monoclonal Antibody [SZ29-01] (ET1603-27)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of Caspase-9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: C2C12 cell lysate
Lane 2: Hela cell lysate

Bcl-XL Recombinant Rabbit Monoclonal Antibody [SZ3-03] (ET1603-28)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Bcl-XL on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

SIRT1 Recombinant Rabbit Monoclonal Antibody [SZ04-01] (ET1603-3)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
Western blot analysis of SIRT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-3, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: HepG2 cell lysate
Lane 2: Jurkat cell lysate

Bax Recombinant Rabbit Monoclonal Antibody [SZ3-07] (ET1603-34)

CITATION

APPLICATIONS

  • WB
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Bax on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-34, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: human liver tissue lysate
Lane 4: PC-12 cell lysate

EGFR Recombinant Rabbit Monoclonal Antibody [SZ40-19] (ET1603-37)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of EGFR on different cell lysates using anti-EGFR at 1/500 dilution.
Positive control: 
Line 1: Hela         
Line 2: A431 
Line 3: HUVEC

AIF Recombinant Rabbit Monoclonal Antibody [SZ05-01] (ET1603-4)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of AIF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: SKOV-3 cell lysate
Lane 2: Hela cell lysate

Phospho-STAT3 (Tyr705) Recombinant Rabbit Monoclonal Antibody [SZ43-01] (ET1603-40)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Phospho-STAT3(Tyr705) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: NIH/3T3 cell lysate
Lane 2: A549 cell lysate
Lane 2: SiHa cell lysate

Phospho-V-Myb+C-Myb (S11) Recombinant Rabbit Monoclonal Antibody [SZ04-81] (ET1603-41)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
ICC staining of P-V-Myb+C-Myb(S11) in AGS cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-41, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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