ERK1/2 Recombinant Rabbit Monoclonal Antibody [SA43-03] (ET1601-29)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
  • ZEBRAFISH
Western blot analysis of ERK1/2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-29, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: SW480 cell lysate
Lane 3: HCT116 cell lysate
Lane 4: PC-12 cell lysate

RPS3 Recombinant Rabbit Monoclonal Antibody [SA46-08] (ET1601-27)

APPLICATIONS

  • WB
  • ICC
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of RPS3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Jurkat cell lysate
Lane 2: PC-12 cell lysate

Filamin A Recombinant Rabbit Monoclonal Antibody [SA30-08] (ET1601-3)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Filamin A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-3, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: MCF-7 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: Hela cell lysate
Lane 4: NIH/3T3 cell lysate

Phospho-Histone H3 (S10) Recombinant Rabbit Monoclonal Antibody [SA31-01] (ET1601-30)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Phospho-Histone H3(S10) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Untreated Hela cell lysate 
Lane 2: Hela cell lysate treated with calyculin A 
Lane 3: Hela cell lysate treated with calyculin A and alkaline phosphatase

Cyclin D1 Recombinant Rabbit Monoclonal Antibody [SA38-08] (ET1601-31)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Cyclin D1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: PC-12 cell lysate
Lane 3: SH-SY5Y cell lysate

GAPDH Recombinant Rabbit Monoclonal Antibody [SA30-01] (ET1601-4)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
  • ZEBRAFISH
Western blot analysis of GAPDH on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFTM/TBST for 1 hour at room temperature. The primary antibody (ET1601-4, 1/10,000) was used in 5% NFTM/TBST at room temperature for 1 hour. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 45 mins at room temperature.
Positive control: 
Lane 1: Rat liver tissue lysate
Lane 2: Rat lung tissue lysate
Lane 3: Rat lung tissue lysate
Lane 4: Rat heart tissue lysate
Lane 5: Rat cerebellum tissue lysate
Lane 6: Rat skeletal muscle tissue lysate
Lane 7: Rat spleen tissue lysate
Lane 8: Rat small intestine tissue lysate

Beta-Catenin Recombinant Rabbit Monoclonal Antibody [SA30-04] (ET1601-5)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Beta-Catenin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-5, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: SW480 cell lysate
Lane 2: HT-29 cell lysate
Lane 3: A431 cell lysate

Cytokeratin 19 Recombinant Rabbit Monoclonal Antibody [SA30-06] (ET1601-6)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of Cytokeratin 19 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Bad Recombinant Rabbit Monoclonal Antibody [SA32-01] (ET1601-7)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Bad on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-7, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody [SA35-03] (ET1601-8)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • RAT
Western blot analysis of Cytokeratin 20 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-8, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: LOVO cell lysate
Lane 2: HCT116 cell lysate
Lane 3: CRC cell lysate

CD9 Recombinant Rabbit Monoclonal Antibody [SA35-08] (ET1601-9)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of CD9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-9, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: mouse heart tissue lysate
Lane 2: Jurkat cell lysate
Lane 3: human lung carcinoma tissue lysate
Lane 4: human lung tissue lysate
Lane 5: MCF-7 cell lysate

Integrin alpha 6 Recombinant Rabbit Monoclonal Antibody [SR45-00] (ET1602-1)

APPLICATIONS

  • WB
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Integrin alpha 6 on Raji cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.

Integrin beta-1 Recombinant Rabbit Monoclonal Antibody [SR30-03] (ET1602-10)

APPLICATIONS

  • WB
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Integrin beta-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-10, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: CRC cell lysate
Lane 2: HepG2 cell lysate

Phospho-Histone (H1.3(T17)+H1.4(T17)) Recombinant Rabbit Monoclonal Antibody [SR38-03] (ET1602-11)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Phospho-Histone H1.3(T17)+Histone H1.4(T17) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Untreated CRC whole cell lysates
Lane 2: CRC cells treated with 1.5ug/ml Colcemid for 12 hours whole cell lysates

NeuN Recombinant Rabbit Monoclonal Antibody [SR45-07] (ET1602-12)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of NeuN on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-12, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: human brain tissue lysate
Lane 2: rat brain tissue lysate
Lane 2: mouse brain tissue lysate

Phospho-Glycogen synthase 1 (S641) Recombinant Rabbit Monoclonal Antibody [SR46-06] (ET1602-13)

APPLICATIONS

  • WB
  • ICC
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of Phospho-Glycogen synthase 1(S641) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-13, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: SK-Br-3 cell lysate
Lane 2: A431 cell lysate

Collagen XVII Recombinant Rabbit Monoclonal Antibody [SR46-05] (ET1602-14)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Collagen XVII on rat skin tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-14, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Rab4A Recombinant Rabbit Monoclonal Antibody [SR27-08] (ET1602-15)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IP

REACTIVITY

  • HUMAN
  • RAT
  • MONKEY
  • MOUSE
Western blot analysis of Rab4A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: MCF-7 cell lysate
Lane 2: 293T cell lysate
Lane 3: COS-1 cell lysate

Cytokeratin 17 Recombinant Rabbit Monoclonal Antibody [SR45-06] (ET1602-16)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
ICC staining of Cytokeratin 17 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-16, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Cdk5 Recombinant Rabbit Monoclonal Antibody [SR39-01] (ET1602-17)

APPLICATIONS

  • WB
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Cdk5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: MCF-7 cell lysate
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