BDNF Recombinant Rabbit Monoclonal Antibody [SJ12-09] (ET1606-42)

APPLICATIONS

  • WB
  • IHC-P
  • ICC
  • IF

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
  • ZEBRAFISH
Western blot analysis of BDNF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-42, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: SHG-44 cell lysate
Lane 2: A172 cell lysate
Lane 3: Mouse brain tissue lysate

PTEN Recombinant Rabbit Monoclonal Antibody [SJ19-03] (ET1606-43)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of PTEN on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-43, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: MCF-7 cell lysate
Lane 2: Hela cell lysate
Lane 3: 293T cell lysate

Phospho-EGFR (Y1092) Recombinant Rabbit Monoclonal Antibody [SJ0194] (ET1606-44)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of Phospho-EGFR(Y1092) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-44, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: A431 cell lysate treated with EGF 
Lane 2: Untreated A431 cell lysate

Met(C-Met) Recombinant Rabbit Monoclonal Antibody [SJ19-05] (ET1606-45)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
Western blot analysis of Met(C-Met) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate

Calmodulin Recombinant Rabbit Monoclonal Antibody [SJ16-09] (ET1606-46)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Calmodulin on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-46, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

LDL Receptor Recombinant Rabbit Monoclonal Antibody [SJ0197] (ET1606-47)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of LDL Receptor on MCF-7 lysates using anti-LDL Receptor antibody at 1/1,000 dilution.

MMP14 Recombinant Rabbit Monoclonal Antibody [SJ18-09] (ET1606-48)

CITATION

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of MMP14 on human kidney tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Integrin beta 3 Recombinant Rabbit Monoclonal Antibody [SJ19-09] (ET1606-49)

APPLICATIONS

  • WB
  • IHC-P
  • ICC
  • IF

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Integrin beta 3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: HUVEC cell lysate
Lane 2: mouse marrow tissue lysate

CD86 Recombinant Rabbit Monoclonal Antibody [SJ20-00] (ET1606-50)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • RAT
  • MONKEY
"Western blot analysis of CD86 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-50, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: Raji cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MCF-7 cell lysate

Musashi 1 Recombinant Rabbit Monoclonal Antibody [SJ201] (ET1606-51)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
ICC staining of Musashi 1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1606-51, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

SUMO-1 Recombinant Rabbit Monoclonal Antibody [SJ20-03] (ET1606-53)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC
  • CHIP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of SUMO-1 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Notch 1 Recombinant Rabbit Monoclonal Antibody [SJ205] (ET1606-55)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of Notch 1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1606-55, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: SW1990 cell lysate
Lane 2: HepG2 cell lysate

Synaptophysin Recombinant Rabbit Monoclonal Antibody [SJ26-85] (ET1606-56)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin at 1/500 dilution.
Positive control:
Lane 1: Human brain
Lane 2: PC-12

CDC7 Kinase Recombinant Rabbit Monoclonal Antibody [SY21-01] (ET1607-1)

APPLICATIONS

  • WB
  • FC

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of CDC7 Kinase on different lysates using anti-CDC7 antibody at 1/1,000 dilution.
Positive control: 
  Lane 1: Jurkat 
  Lane 2: Hela

Apaf-1 Recombinant Rabbit Monoclonal Antibody [SY22-02] (ET1607-12)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P

REACTIVITY

  • HUMAN
  • MOUSE
Western blot analysis of Apaf-1 on HUVEC cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-12, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Caveolin-2 Recombinant Rabbit Monoclonal Antibody [SY22-05] (ET1607-15)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
Western blot analysis of caveolin-2 on HUVEC cell lysates using anti-caveolin-2 antibody at 1/1,000 dilution.

Caveolin-3 Recombinant Rabbit Monoclonal Antibody [SY22-06] (ET1607-16)

APPLICATIONS

  • WB
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Caveolin-3 on different lysates using anti-Caveolin-3 antibody at 1/1,000 dilution.
Positive control:   
Lane 1: Mouse heart      
Lane 2: Mouse skeletal muscle

CD63 Recombinant Rabbit Monoclonal Antibody [SY21-02] (ET1607-2)

CITATION

APPLICATIONS

  • WB
  • IHC-P

REACTIVITY

  • HUMAN
Western blot analysis of CD63 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HL-60 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: NIH/3T3 cell lysate

Phospho-PKR (T446) Recombinant Rabbit Monoclonal Antibody [SY230] (ET1607-20)

APPLICATIONS

  • WB
  • IHC-P
  • IP
  • CHIP

REACTIVITY

  • HUMAN
Western blot analysis of Phospho-PKR(T446) on different lysates using anti-Phospho-PKR(T446) antibody at 1/500 dilution.
Lane 1: Hela treated with Calyculin A and TNF-alpha whole cell lysates 
Lane 2: Untreated Hela whole cell lysates

Paxillin Recombinant Rabbit Monoclonal Antibody [SY23-02] (ET1607-22)

APPLICATIONS

  • WB
  • ICC
  • IF
  • IHC-P
  • IP

REACTIVITY

  • HUMAN
  • MOUSE
  • RAT
Western blot analysis of Paxillin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-22, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control: 
Lane 1: NIH/3T3 cell lysate
Lane 2: A549 cell lysate
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