Anti-Body

cl-2 Rekombinant Tavşan Monoklonal Antikor [SZ10-03] (ET1603-11)

ALINTI

UYGULAMALAR

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ICC
EĞER
IHC-P
IP
FC

REAKTİVİTE

İNSAN
FARE

Western blot analysis of Bcl-2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: U937 cell lysate
Lane 3: THP-1 cell lysate

NF-kB p65 Rekombinant Tavşan Monoklonal Antikor [SZ10-04] (ET1603-12)

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UYGULAMALAR

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ICC
EĞER
IHC-P
IP
FC

REAKTİVİTE

İNSAN
FARE
ZEBRA BALIĞI

Western blot analysis of NF-κB p65 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-12, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: K562 cell lysate
Lane 1: THP-1 cell lysate
Lane 1: MCF-7 cell lysate
Lane 2: Hela cell lysate

ATF2 Rekombinant Tavşan Monoklonal Antikor [SZ17-01] (ET1603-15)

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ICC
EĞER
IHC-P
IP

REAKTİVİTE

İNSAN

ICC staining ATF2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fosfo-eIF-2a (S51) Rekombinant Tavşan Monoklonal Antikor [SZ01-06] (ET1603-14)

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ICC
EĞER
IHC-P
IP
FC

REAKTİVİTE

İNSAN
FARE
FARE

Fosfo-eIF-2a(S51)'in farklı lizatlar üzerinde Western blot analizi. Proteinler bir PVDF membranına aktarıldı ve oda sıcaklığında 1 saat boyunca PBS içinde %5 BSA ile bloke edildi. Birincil antikor (ET1603-14, 1/500), 2 saat boyunca oda sıcaklığında %5 BSA içinde kullanıldı. Keçi Anti-Rabbit IgG - HRP Sekonder Antikoru (HA1001) 1:5,000 seyreltmede oda sıcaklığında 1 saat kullanıldı. Pozitif kontrol: Şerit 1: HUVEC hücre lizatı Şerit 2: Hela hücre lizatı Şerit 2: PC-3M hücre lizatı

Kaspaz-8 Rekombinant Tavşan Monoklonal Antikor [SZ01-08] (ET1603-16)

ALINTI

UYGULAMALAR

Dünya Bankası
ICC
EĞER
IHC-P

REAKTİVİTE

İNSAN

Western blot analysis of Caspase-8 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-16, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: K562 cell lysate

NFkB p105/p50 Rekombinant Tavşan Monoklonal Antikor [SZ20-01] (ET1603-18)

UYGULAMALAR

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IHC-P

REAKTİVİTE

İNSAN
FARE
FARE

Western blot analysis of NFkB p105/p50 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: PC-12 cell lysate

Tau Rekombinant Tavşan Monoklonal Antikor [SZ03-03] (ET1603-2)

UYGULAMALAR

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IHC-P
IP

REAKTİVİTE

FARE
FARE
İNSAN

Western blot analysis of Tau on mouse brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-2, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

MEK1 Rekombinant Tavşan Monoklonal Antikor [SZ22-01] (ET1603-20)

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ICC
EĞER
IHC-P
IP
FC

REAKTİVİTE

İNSAN
FARE
FARE
İNEK
KÖPEK

Western blot analysis of MEK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A431 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Hela cell lysate

Fosfo-Erk1(T202)+Erk2(T185) Rekombinant Tavşan Monoklonal Antikor [SZ2-4] (ET1603-22)

UYGULAMALAR

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ICC
IHC-P
FC

REAKTİVİTE

İNSAN

ICC staining of Phospho-Erk1(T202)+Erk2(T185) in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-22, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

ERK2 Rekombinant Tavşan Monoklonal Antikor [SZ25-01] (ET1603-23)

UYGULAMALAR

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ICC
EĞER
IHC-P
IP
FC

REAKTİVİTE

İNSAN
FARE
FARE

Western blot analysis of ERK2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-23, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: PC-12 cell lysate
Lane 2: Hela cell lysate

Phospho-c-Myc (T58+S62) Rekombinant Tavşan Monoklonal Antikor [SZ02-06] (ET1603-24)

UYGULAMALAR

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ICC
EĞER
IP

REAKTİVİTE

İNSAN
FARE

Western blot analysis of Phospho-c-Myc (T58+S62) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: K562 cell lysate
Lane 2: SH-SY5Y cell lysate

pro Kaspaz-3 Rekombinant Tavşan Monoklonal Antikor [SZ02-08] (ET1603-26)

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ICC
EĞER
IHC-P
IP
FC

REAKTİVİTE

İNSAN
FARE

Western blot analysis of pro Caspase-3 on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Kaspaz-9 Rekombinant Tavşan Monoklonal Antikor [SZ29-01] (ET1603-27)

ALINTI

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ICC
EĞER
IHC-P
IP

REAKTİVİTE

İNSAN
FARE

Western blot analysis of Caspase-9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: C2C12 cell lysate
Lane 2: Hela cell lysate

Bcl-XL Rekombinant Tavşan Monoklonal Antikor [SZ3-03] (ET1603-28)

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ICC
EĞER
IHC-P
IP
FC

REAKTİVİTE

İNSAN
FARE
FARE

Western blot analysis of Bcl-XL on Jurkat cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

SIRT1 Rekombinant Tavşan Monoklonal Antikor [SZ04-01] (ET1603-3)

UYGULAMALAR

Dünya Bankası
ICC
EĞER
IHC-P
IP

REAKTİVİTE

İNSAN

SIRT1'in farklı lizatlar üzerinde Western blot analizi. Proteinler bir PVDF membranına aktarıldı ve oda sıcaklığında 1 saat boyunca PBS içinde %5 BSA ile bloke edildi. Birincil antikor (ET1603-3, 1/500), 2 saat boyunca oda sıcaklığında %5 BSA içinde kullanıldı. Keçi Anti-Rabbit IgG - HRP Sekonder Antikoru (HA1001) 1:5,000 seyreltmede oda sıcaklığında 1 saat kullanıldı. Pozitif kontrol: Şerit 1: HepG2 hücre lizatı Şerit 2: Jurkat hücre lizatı

Bax Rekombinant Tavşan Monoklonal Antikor [SZ3-07] (ET1603-34)

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IHC-P
IP
FC

REAKTİVİTE

İNSAN
FARE
FARE

Bax'ın farklı lizatlar üzerinde Western blot analizi. Proteinler bir PVDF membranına aktarıldı ve oda sıcaklığında 1 saat boyunca PBS içinde %5 BSA ile bloke edildi. Primer antikor (ET1603-34, 1/500), 2 saat boyunca oda sıcaklığında %5 BSA içinde kullanıldı. Keçi Anti-Rabbit IgG - HRP Sekonder Antikoru (HA1001) 1:5,000 seyreltmede oda sıcaklığında 1 saat kullanıldı. Pozitif kontrol: Şerit 1: Hela hücre lizatı Şerit 2: MCF-7 hücre lizatı Şerit 3: insan karaciğer doku lizatı Şerit 4: PC-12 hücre lizatı

EGFR Rekombinant Tavşan Monoklonal Antikoru [SZ40-19] (ET1603-37)

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ICC
EĞER
IHC-P
IP

REAKTİVİTE

İNSAN
FARE
FARE

1/500 seyreltmede anti-EGFR kullanılarak farklı hücre lizatları üzerinde EGFR'nin Western blot analizi. Pozitif kontrol: Hat 1: Hela Hat 2: A431 Hat 3: HUVEC

AIF Rekombinant Tavşan Monoklonal Antikor [SZ05-01] (ET1603-4)

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ICC
IHC-P
IP
FC

REAKTİVİTE

İNSAN
FARE
FARE

Farklı lizatlar üzerinde AIF'nin Western blot analizi. Proteinler bir PVDF membranına aktarıldı ve oda sıcaklığında 1 saat boyunca PBS içinde %5 BSA ile bloke edildi. Birincil antikor (ET1603-4, 1/500), 2 saat boyunca oda sıcaklığında %5 BSA içinde kullanıldı. Keçi Anti-Rabbit IgG - HRP Sekonder Antikoru (HA1001) 1:5,000 seyreltmede oda sıcaklığında 1 saat kullanıldı. Pozitif kontrol: Şerit 1: SKOV-3 hücre lizatı Şerit 2: Hela hücre lizatı

Phospho-STAT3 (Tyr705) Rekombinant Tavşan Monoklonal Antikor [SZ43-01] (ET1603-40)

ALINTI

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ICC
EĞER
IHC-P
IP

REAKTİVİTE

İNSAN
FARE
FARE

Western blot analysis of Phospho-STAT3(Tyr705) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: NIH/3T3 cell lysate
Lane 2: A549 cell lysate
Lane 2: SiHa cell lysate

Phospho-V-Myb+C-Myb (S11) Rekombinant Tavşan Monoklonal Antikor [SZ04-81] (ET1603-41)

UYGULAMALAR

Dünya Bankası
ICC
EĞER
IHC-P
FC

REAKTİVİTE

İNSAN

AGS hücrelerinde (yeşil) PV-Myb+C-Myb(S11)'in ICC boyaması. Formalin ile sabitlenmiş hücreler, oda sıcaklığında 10 dakika süreyle TBS içinde %0.1 Triton X-100 ile geçirgenleştirildi ve oda sıcaklığında 15 dakika süreyle %1 Bloker BSA ile bloke edildi. Hücreler, oda sıcaklığında 1 saat süreyle birincil antikor (ET1603-41, 1/50) ile incelendi, PBS ile yıkandı. Alexa Fluor®488 Keçi anti-Rabbit IgG, 1/1.000 seyreltmede ikincil antikor olarak kullanıldı. Nükleer karşı leke DAPI'dir (mavi).

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